Posts – Nazmul Alam

(U)HPLC definition and principles

Hey there, let’s talk about high-performance liquid chromatography, or HPLC for short. It’s a fancy way of saying that we use a machine to separate and analyze (identify…

Binary versus Quarternary Pumps in (U)HPLC Systems

Hey there! Let’s talk about the different ways scientists separate compounds in liquid chromatography (LC). There are two main methods: isocratic and gradient.

FDA Bioanalytical method validation guidlines- summary

This is based based on the original FDA guideline

HPLC Separations: Isocratic vs. Gradient

When it comes to HPLC separations, there are two modes: isocratic and gradient. Isocratic elution refers to a constant concentration of the mobile phase throughout the…

The Colorful History and Evolution of Liquid Chromatography: From Tswett to UPLC

The history of liquid chromatography dates back to the early 1900s when Russian botanist Mikhail Semyonovich Tswett invented a method for separating plant pigments using…

The Impact of Dwell Volume in (U)HPLC

If you’re into (U)HPLC, then you know how frustrating it can be to transfer a gradient method between instruments and end up with inconsistent results. One of the most…

Types of HPLC pumps based on flow rates

Here are some of the most common types of HPLC pumps based on flow rates:

Understanding (U)HPLC Pumps: Piston vs Plunger and Cam vs Crankshaft Driven Mechanisms

Pumps are mechanical devices that transfer mobile phases from low-pressure reservoirs to high-pressure areas in the system. Their primary function is to maintain a constant…

Understanding Single-Piston vs. Dual-Piston Pumps in HPLC Systems

The basic design of a pump for early LC systems featured a single piston or plunger in the pump head, making it a single-piston/plunger pump. Its flow rate was controlled by…